FastQCFastQC Report
Thu 28 Apr 2016
ERR1042308.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042308.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5242976
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28700.021.6585371
ATTAGAC4250.020.4588243
TTAGACT4700.017.3191494
CTAGTAC2950.015.6779663
TATTAGA7400.015.52
TAGACTA6250.014.85
TTATAGT3300.014.5757594
GTATTAG13150.014.349811
ATAATAC7200.014.1319453
TCTAGCG4150.013.81927728
TTAACGG2151.9826984E-1013.76744235
GTACTAG3250.013.6615381
TTTATAC4750.013.6315783
CGGGTAA4500.013.56666724
CTATTAG8350.013.514971
GTATCAA45700.013.4398242
CTTATAC9550.013.36649137
TCTTATA13400.013.25373237
TAATACG6100.013.0409834
AATACGC6100.013.0409835
Produced by FastQC (version 0.11.5)