FastQCFastQC Report
Thu 28 Apr 2016
ERR1042307.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042307.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9357458
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT111580.11924178553620012No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57600.023.0286461
TTAGACT10500.015.6809524
GTATCAA92150.014.1334782
TAGACTA9500.013.8263165
TATACTG12000.013.7208335
CTAGTAC6150.013.5365853
ATAATAC14050.013.2989333
ATTAGAC9150.013.1420773
TCGCTAA6850.012.96350414
TTAGCGA8000.012.71874927
TATTAGA13550.012.6974172
GTATTAT13800.012.1992761
TAATACT16050.011.8722744
ATTAGAG12000.011.8708323
ATACCGC14200.011.85563427
TCGTTTA8150.011.8036830
GTATTAG21800.011.7958721
CGATCGC7700.011.77272819
TAATACG9900.011.7727274
CGCGATA1100.00374847411.77272714
Produced by FastQC (version 0.11.5)