FastQCFastQC Report
Thu 28 Apr 2016
ERR1042305.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042305.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3169673
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT60310.19027199335704345No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT58250.18377290023292625No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT49480.15610443096180585No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT41470.13083368536754422No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC39690.12521796412437497No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC39400.12430304324767885No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT37170.11726761719584324No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA37060.11692057824261366No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC32370.10212409923673514No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGA5600.018.16964116
GACGGAC9800.018.122457
ACGGACC9900.017.9393948
AAGACGG10800.017.4722235
CGCAAGA11150.016.4260082
TCTAGCG8450.016.42011828
AGACGGA11350.016.299566
TCTAATA3550.016.154932
CGGACCA11000.016.1454549
TAACGAA6350.016.02362313
ACGAACG6300.015.85714215
ATAACGA6450.015.77519312
TTAACGG1659.767973E-1015.6969735
CTAGCGG8850.015.46892729
GCGCAAG12100.015.4421481
GTTCAAA13100.015.2519081
CAAGACG13100.015.1106874
TATTAGA4250.014.82
CGAGCCG12300.014.43902415
TAATAGT3350.014.3582094
Produced by FastQC (version 0.11.5)