FastQCFastQC Report
Thu 28 Apr 2016
ERR1042304.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042304.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3128478
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17050.017.5777131
TTAATAC2850.015.5789483
CGTATTA1802.0190782E-1015.41666615
GTATTAG6750.015.3481481
TATAACG855.367818E-415.2352942
TATTAGA2900.014.0344842
GTGTAAG6300.013.8015871
TAATACT4050.013.7037044
GTATAAA3000.013.5666671
TAGGACA3300.013.4545454
TTAACGG1254.8495953E-513.3235
TCTATAC1951.0279109E-813.2820523
GTATTAT3500.013.2142861
TTACTCG2254.0745363E-1013.15555619
TTATACC3250.013.0923074
CTTACAC4100.013.0853663
AATACTG5800.013.0775875
GCGTTAT3700.013.01
TAGACTG3600.012.8472225
TTAGACT2602.3646862E-1112.8076934
Produced by FastQC (version 0.11.5)