FastQCFastQC Report
Thu 28 Apr 2016
ERR1042303.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042303.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2091308
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT28190.13479602239364072No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC25430.1215985402437135No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC24940.1192555089924583No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT22930.10964429916588087No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA21270.1017066830902No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT21130.1010372455898414No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAAT450.003826840320.5555571
ATTAGAG3950.019.6708853
TTAGAGT4050.018.7283954
TAACGAA3650.017.23287813
TAGACCG650.001580519317.0769235
ACGGACC7200.016.9583348
AAGACGG8300.016.9397585
AGACGGA8000.016.881256
GACGGAC7350.016.8639477
CGAACGT2900.016.5862064
TAACCTA2350.016.5319145
CGGACCA7450.016.3892619
TTTATAC1708.54925E-1116.3235283
CTATTAG1951.8189894E-1216.1282061
ATAACGA3900.016.12820612
CGCAAGA7950.016.0566022
TACCGTC4150.016.0481937
CGTATCG700.002593446315.85714215
TTAGACT1557.21775E-915.5161294
TATTAGA2400.015.4166672
Produced by FastQC (version 0.11.5)