FastQCFastQC Report
Thu 28 Apr 2016
ERR1042302.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042302.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8691356
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT158090.18189336623652283No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT130240.14985003490824678No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT107960.12421536984562594No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA91750.016.8768391
TCGTTTA14650.016.41638230
ACGGACC21500.015.6604668
TTAGAGT13400.015.1865674
TTAACGG5300.015.00943435
GACGGAC22500.014.8822227
ATAACGC18750.014.7999993
TAACGCC17250.014.5855064
TAATACT18400.014.4782614
TAATACG6800.014.4191184
TAACGGC5400.014.38888936
CGTTTAT17050.014.10557231
AATAACG17500.014.062
CGCATCG17800.014.030913
TTAGACT8450.013.79294
ATTAGAG18800.013.6781923
TCTATAC8200.013.5365853
CGCAAGA24950.013.494992
TAGACCG1805.1721145E-813.3611125
CGGACCA26000.013.3057699
Produced by FastQC (version 0.11.5)