FastQCFastQC Report
Thu 28 Apr 2016
ERR1042301.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042301.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5269220
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT291590.553383612754829No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT247490.469690011045278No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT235380.4467074823218617No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100280.1903127977195866No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATA601.338929E-624.66666814
TATTAGA8150.017.2515352
GGTATCA125450.017.1359121
TAACGCC8700.017.0114944
ATTAGAC4700.016.5319163
TTAGACT5750.015.7652184
GTATTAG13200.015.4166671
TCGTTTA8150.015.208590530
GAATAAC10000.014.7999991
AATAACG8650.014.7572252
TAGACTA5400.014.7314825
TTAACGG2900.014.67241435
GTACTAG2800.014.5357141
CGAACGA7350.014.34693916
ATAACGC9800.014.3469383
TATACTG6300.014.0952385
CGCATCG8950.014.05586513
ATTAGAG7650.014.0261443
TCTAGAC3450.013.9420283
TAATACT9600.013.8750014
Produced by FastQC (version 0.11.5)