FastQCFastQC Report
Thu 28 Apr 2016
ERR1042300.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042300.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6857689
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT293880.42854086850541045No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT262740.38313198513376734No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT205770.3000573516821775No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90680.13223113500772637No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA91600.021.8930131
TACCGTC11800.016.4618647
AAGACGG19650.016.1933845
ACCGTCG12250.015.5551028
ATTAGAG12300.015.491873
CCGTCGT11950.015.4811729
TTAGAGT9500.015.3842114
ACGGACC19250.015.3766228
ATACCGT14650.015.2798636
CGCAAGA19900.014.9673372
GTATCAA135500.014.78634552
CGTCGTA11650.014.76824110
GACGGAC20150.014.6898267
CAAGACG24450.014.3006134
AGACGGA21650.014.1847586
TCTATAC5900.013.796613
GTATACG3150.013.50793651
CGAAAGC21550.013.30626519
CGGACCA22100.013.2262459
GCGAAAG22000.013.20227218
Produced by FastQC (version 0.11.5)