FastQCFastQC Report
Thu 28 Apr 2016
ERR1042299.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042299.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3005498
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT45730.15215448488070862No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT35180.117052149094759No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT33990.11309273870752866No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16100.026.7732931
TAGACTA3200.021.3906275
TTAGACT3250.020.4923084
ATTAGAC3050.019.4098363
TATTAGA4200.017.6190472
CTAGTAC1902.7284841E-1115.5789483
TTAGCGA3850.015.37662327
GTATACG1101.4533418E-515.1363641
GTATCAA29350.015.12776851
TAATAGT5400.014.7314814
GTATTAT3950.014.0506341
CGAACGT1203.3038454E-513.8750014
CTATTAG4550.013.8241751
GTCCTAA4350.013.6091961
GTCTAGA2501.0913936E-1113.321
GTACCTA1409.570294E-613.2142851
GTACTAG1409.570294E-613.2142851
ATAATAC3250.013.0923073
GTATTAG5100.013.0588241
TATATCG1000.001833677912.9499995
Produced by FastQC (version 0.11.5)