FastQCFastQC Report
Thu 28 Apr 2016
ERR1042297.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042297.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10512836
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT565870.5382657924084424No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT561610.5342136032560576No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT362530.3448451017403867No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG303380.2885805504813354No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC278450.26486668297688654No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT241430.22965258851179643No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTT207880.1977392208914892No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG205660.1956275166853169No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG192670.18327119342487602No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC191670.18231997531398758No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG190710.1814068059275347No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC189570.18032241728112186No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT178890.17016340785683332No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA164430.15640879397338645No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG150970.14360539820082802No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGAC147830.1406185733326383No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG145170.13808833315767505No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC140110.13327516951657956No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGA139110.1323239514056911No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA131630.12520883993624557No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCA129840.12350615951775525No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT128980.1226881119423912No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAAC123180.11717104689923823No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC122950.11695226673373389No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC115120.10950422892547738No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG114930.10932349748440859No Hit
GGCTTATGCGGAGGAGAATGTTTTCATGTTACTTATACTAACA112750.10724984200267179No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT112570.10707862274271186No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCAT111810.10635569697843665No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT111640.1061939898995856No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGAGT21750.018.797712
CCGAGTT23050.017.89804813
CTCTAAT72500.017.3262061
GGGTAAG26350.017.060721
AATACTG77650.016.7488735
TTGCCGA25550.016.29158410
GGTAAAC74500.016.28993435
GGGGTTA54400.016.2555146
GTAAACA76400.015.9574636
TTAACGG65000.015.93846135
CGTATTA12900.015.91860515
GAGTTCC25950.015.89788115
ATACTGG82350.015.8828166
GTGACAC67500.015.8688924
GTAAGAT28000.015.8571423
TAACGGC65350.015.853136
TACTGGT81650.015.7924067
TCTAATA86400.015.7806722
TAAACAG77300.015.77166937
TGCCGAG26450.015.73724111
Produced by FastQC (version 0.11.5)