FastQCFastQC Report
Thu 28 Apr 2016
ERR1042296.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042296.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8874092
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC264130.2976417192880128No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG245190.2762986906153328No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT208530.2349874218117189No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT197560.2226255936945436No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG187020.21074832219454115No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC184040.2073902321499484No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG178910.2016093590194918No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC176080.1984203003529826No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTT162290.18288068232783702No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG159930.18022125531265623No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGAC151780.17103721710345127No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT151120.17029347903988373No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA149380.16833271505411485No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG133560.1505055390455722No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCA132490.14929978188190973No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG131370.14803768092555272No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC129830.14630229211056184No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT127650.14384570274908126No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT126270.1422906140707128No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC122100.13759154175999078No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGA108810.1226153616617903No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAAC108600.12237871773247337No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA107950.12164624842744476No Hit
GATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTT104740.11802897693645727No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG103520.11665418839471127No Hit
ATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCA101690.11459200558209222No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT100160.11286788552564025No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT94670.10668133708778317No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC94640.10664753081216648No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATG93140.1049572170313312No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGG93090.1049008732386367No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG92990.10478818565324767No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG92500.10423601648484149No Hit
GTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTT89850.10124979547203251No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG89590.10095680775002107No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC89000.10029195099622587No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGAGT17900.018.60335212
CCGAGTT18700.017.60962713
TAGGACC28600.017.2709794
CTCTAAT56600.017.192581
GGGGTTA42400.017.0601436
GTGACAC61400.016.66205224
AATACTG61500.016.4243895
TTAACGG60950.016.26907335
GGTAAAC57300.016.17539235
TAACGGC61000.016.16475536
CACATGT63950.016.142328
TATTAGA70900.016.125532
ATTAGAG65450.016.0267373
GTAAGAT21950.016.0136683
GGGTAAG22350.015.9753921
ATACTGG61550.015.9601956
GGGTTAG44900.015.9454357
AACGGCC62400.015.86137937
GACACAT64900.015.84899826
AGTGACA65000.015.824615523
Produced by FastQC (version 0.11.5)