FastQCFastQC Report
Thu 28 Apr 2016
ERR1042294.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042294.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10183308
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT674630.662486099801754No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT511370.5021649153693476No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT324260.31842305074146826No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG295580.2902593145567236No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC271410.2665243946269719No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT236960.23269452323351117No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC211770.2079579641507455No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG208900.20513962653393178No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC186180.18282860540013127No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTT185920.18257328561602967No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG177300.17410845277389234No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT168410.16537848015595716No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA164650.16168616327818033No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG162610.15968288497215247No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG151910.1491754938572024No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGA148910.14622949634833787No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC146010.14338169875643553No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT141750.13919838229384793No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGAC139210.1367041044030093No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG128720.12640293311367976No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG126560.12428181490729731No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCAT124800.12255349636876348No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT121750.1195583989014179No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT120120.11795774025493484No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG119090.1169462811102247No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAAC117410.11529652250526057No Hit
GGCTTATGCGGAGGAGAATGTTTTCATGTTACTTATACTAACA116460.11436362329412014No Hit
CCTAAGAACAGCTAAAAGAGCACACCCGTCTATGTAGCAAAAT116180.11408866352662612No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC115920.11383334374252455No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCA114640.11257638480540902No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACA109240.1072735892894529No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCA108530.10657636987902164No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA106080.10417047191344894No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT104560.10267783317562426No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC103560.10169583400600275No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTT19200.019.6562513
GCCGAGT19450.019.30848312
CTCTAAT67050.017.382551
GGGTAAG24200.017.2768611
TTAACGG60300.017.11940435
TAACGGC60350.017.07456436
GTGACAC62750.016.83426324
AACGGCC62500.016.605637
CACATGT64300.016.42846128
GTAAGAT24550.016.4276983
TTGCCGA23950.016.29853810
AATACTG76000.016.18755
GACACAT65550.016.08695626
TTTAACG65550.015.94584234
GGGGTTA49150.015.846396
CTAATAC86350.015.8112333
TGACACA67550.015.77498125
ATACTGG76750.015.7641696
AGTGACA67600.015.76331423
GTCGCCC57650.015.75628937
Produced by FastQC (version 0.11.5)