FastQCFastQC Report
Thu 28 Apr 2016
ERR1042293.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042293.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10555067
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT671060.6357704787662646No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT545160.5164912738119047No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT346040.32784254235430244No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT249720.23658779238445385No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG239060.22648837757259146No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC220640.20903704353558342No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC177560.16822252288876993No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG156280.14806158975589637No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC156020.14781526256536315No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG149430.1415718156976171No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG148240.14044439509479192No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT143230.13569785961567085No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTT142530.13503467102577368No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA133660.12663112417950545No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGAC117090.1109325028443685No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC116340.11022194364090725No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG114010.108014473048821No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGA113640.1076639305084468No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT109720.10395007440502273No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG109140.10340057528767936No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG51400.017.67217835
TAACGGC52200.017.25957936
GCCGAGT17600.017.23863612
CCGAGTT17750.016.88450813
CTCTAAT59400.016.880471
GTGACAC56700.016.44444524
AACGGCC55300.016.39240537
TTTAACG57100.016.1672534
AATACTG67500.016.0881485
ATTAGAG60050.015.9583693
TCTAATA69700.015.9253952
GGGTAAG22450.015.9042311
CACATGT59300.015.81703228
GTATTAG73000.015.8136981
GACACAT59650.015.75523926
TATTAGA67000.015.7111942
TAATACT78700.015.6086414
CTAATAC76850.015.5269993
GGTAAAC61350.015.37897335
ATACTGG68100.015.3487526
Produced by FastQC (version 0.11.5)