FastQCFastQC Report
Thu 28 Apr 2016
ERR1042292.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042292.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10315910
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT827410.8020717513045384No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG538550.5220576759587859No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT475470.4609094108033125No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG334430.324188559225507No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT313540.3039382856190099No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG295170.2861308406141581No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT293020.2840466812913257No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT275310.2668790247297621No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTT229910.22286933484297555No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG228560.22156067666352264No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG219850.21311740796497838No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC200820.19467017451683855No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAG191660.1857906864251433No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTA180990.17544743992531925No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC175380.17000923815737048No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGT166000.16091648725124588No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTG164180.15915222215005753No Hit
AAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAA149530.14495085746192046No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG141540.13720553979241773No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG139600.13532494951972246No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC137570.13335711536839695No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT133980.12987705398748148No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA129660.12568934781323218No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGT121420.11770168603642335No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTT121350.11763382968637764No Hit
ACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGA121240.11752719827916297No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG119530.11586956458518928No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGAC103730.1005534170034442No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG5300.017.8018881
CTCTAAT50150.017.3379881
GGGGTTA40550.017.2453776
TTAACGG46000.016.8108735
GGGTTAG40900.016.735947
TAACGGC46450.016.60818136
AATACTG56350.016.415265
CTAGTAC6750.016.170373
GTCGTCA5750.016.08695624
CCGAGTT14650.016.03754213
AACGGCC48350.016.03205737
TTTAACG50000.015.91000134
TAGGTCG5400.015.75925921
ATACTGG57900.015.7521586
ATTAGAG52400.015.6755733
TCGCTAA4500.015.62222214
CACATGT51100.015.56751528
TATTAGA59750.015.54309752
GTGACAC50550.015.51730924
GTCGCCC42100.015.46793337
Produced by FastQC (version 0.11.5)