FastQCFastQC Report
Thu 28 Apr 2016
ERR1042291.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042291.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10007244
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG1084571.0837849062139386No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1028691.0279453563838354No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG641650.6411855252055412No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG627250.6267959490145338No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT603290.6028532930744969No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT538200.5378104101388953No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTT494990.4946316888046299No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG426890.4265809847346582No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAG373880.37360935738151285No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT367380.36711406257307205No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTA340450.34020355654363976No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGT336330.3360865389112127No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT334770.33452766815718693No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTG327340.327103046553077No Hit
AAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAA279960.27975734378016565No Hit
GCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTG247120.24694111585567413No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGT239400.23922670417549527No Hit
ACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGA224380.22421757678737522No Hit
CAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGC190740.19060192796338332No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTG188880.18874327437204488No Hit
GCTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCG188340.1882036652648821No Hit
ACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATAC183890.18375688651141114No Hit
GTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAG177100.1769718016269015No Hit
GGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGT163910.1637913495463886No Hit
TATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTAC150120.15001133179125042No Hit
CCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTA135890.13579163254138701No Hit
GTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCT123700.12361045658524963No Hit
GCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCACTGC122960.12287099225321177No Hit
GCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACG119140.11905375745809735No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGCT6350.017.77165430
CTCTAAT22200.016.3333341
TTAACGG17850.016.06442535
CTAGTAC8450.015.7633123
ATTAGAG22050.015.68934253
TAATACT34500.015.5507264
GTACTAG5000.015.541
ACGCTAT6950.015.438848532
TAACGGC18650.015.37533536
TACGCTA7000.015.06428631
CTAATAC33550.014.8330843
AATACTG30150.014.7263675
CGAGACG8850.014.63276823
TTAGACT7250.014.54482654
GTATTAG34650.014.5223661
GGGGTTA18950.014.4485496
TATTAGA27650.014.3182642
TTTAACG21200.014.31132134
CGTATTA9900.014.20202115
TCTAATA33050.014.1059012
Produced by FastQC (version 0.11.5)