FastQCFastQC Report
Thu 28 Apr 2016
ERR1042290.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042290.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7692717
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1098111.4274670444785633No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT741490.9638857116412834No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG580320.7543758596605075No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT512530.6662535486486764No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT395710.5143956290085804No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG354920.4613714504251229No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG309920.40287456304450037No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT276940.3600028442486575No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTT262140.34076386795458613No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAG197860.25720431415844364No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG184280.23955125347780246No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTA178400.23190766019340112No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGT161630.21010782016288912No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTG149310.19409267232890537No Hit
AAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAA132170.1718118578910416No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGT125890.16364829227436808No Hit
GCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTG125730.1634403033414592No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTG120220.15627768446440965No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA120170.15621268792287563No Hit
ACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGA115760.15047999295957462No Hit
GTACGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA106590.13855962724223445No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95780.12450737496257824No Hit
CAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGC95260.12383141093062439No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT93770.12189451399291044No Hit
ACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATAC93490.1215305333603199No Hit
GTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAG92550.12030859837948024No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91120.11844969729160712No Hit
GCTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCG85760.11148206803915965No Hit
CCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTA85750.11146906873085283No Hit
GGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGT79470.10330550311417928No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77290.10047165390329582No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGCT7850.018.38216630
GACGCTA8850.017.76836226
TTAACGG9900.017.75252535
CGAGACG9800.016.98979823
CTAGTAC6800.016.051473
CGCTACG9550.015.88481628
ACGCTAT9850.015.7766532
ACGCTAC9800.015.66836827
TAACGGC11350.015.1585936
ATTAGAG13550.015.1549813
GTACTAG5050.015.0198011
ATCTCGT10250.014.98048937
TATACAC16800.014.8660713
CTACGCG1001.0946498E-414.7999999
GCTACGC10300.014.72815629
TACGCTA9900.014.57575831
ACGAGAC11700.014.54700922
CCCACGA12550.014.44621519
CGCTATC10800.014.21759333
GTATTAG21400.014.1775711
Produced by FastQC (version 0.11.5)