FastQCFastQC Report
Thu 28 Apr 2016
ERR1042288.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042288.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12287000
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1713411.3944901114999593No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1241301.0102547407829412No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT848690.6907219011963864No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT578340.47069260193700657No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG296390.2412224302107919No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG176880.14395702775290958No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA167030.135940424839261No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG166430.13545210384959713No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT158760.12920973386506063No Hit
GTACGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA142920.11631805973793441No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG138750.11292422885977049No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC130680.10635631154879141No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC127150.10348335639293563No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTT126480.1029380646211443No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126460.10292178725482216No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGT12800.016.76562537
CGAGACA13700.015.7992723
TTAACGG36700.015.67711335
TAACGGC37250.015.44563736
ATTAGAG42750.015.232753
TATTAGA47150.015.1845182
CCCACGA14350.015.08362319
GTATTAG62900.014.85294151
GGGGTTA30550.014.2913266
TAGGGGT30650.014.24469954
TCTAATA47550.014.2008412
TAATACT54100.014.1571164
AACGGCC41700.014.15227837
CCGAGTT13250.014.10188813
ACGAGAC15400.014.05519422
AATACTG47150.013.8504775
CTCTAAT42450.013.7714961
AATCTCG16550.013.74924436
GGTAAAC39400.013.7106635
GTGACAC43750.013.61600124
Produced by FastQC (version 0.11.5)