FastQCFastQC Report
Thu 28 Apr 2016
ERR1042287.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042287.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10627708
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1315621.2379150800906462No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT955100.8986885977672702No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT691350.6505165554040439No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG544360.5122082767046291No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT457890.43084548427563124No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG316390.2977029478039856No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG295000.2775763127854096No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTT216460.20367514801874495No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAG169110.15912179747505295No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTA156810.14754827663688164No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT141220.13287907420866288No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT122270.11504832462465096No Hit
AAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAA115530.10870641157999447No Hit
ACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGA111940.10532844899389408No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA110800.10425578120889284No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGT11500.016.73043437
GTCGGGA10400.016.0096152
TTAACGG23400.015.49572735
AATACTG35050.014.6205435
GGTAAAC27800.014.57374235
TAACGGC24600.014.51422836
TAATACT44050.014.4052224
GTATTAG50250.014.3213941
ATACTGG35100.014.1253576
GAGACCG13500.014.11481624
CCCACGA14500.014.03448319
AGACCGA13300.013.77067825
CCGAGTT9450.013.70370413
TACTGGT33650.013.5794957
TTGGTAA30950.013.44911233
CCGTCGT3900.013.282059
GACCGAG14650.013.25938626
TAGGACC27500.013.1854554
ATCTCCG14600.013.17808210
TATTAGA36800.013.1711962
Produced by FastQC (version 0.11.5)