FastQCFastQC Report
Thu 28 Apr 2016
ERR1042286.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042286.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7996821
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT637230.7968541499178237No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT453530.5671378664096646No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT361330.45184205073491074No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT336430.42070467752123No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101800.12730058607038972No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86170.1077553192699949No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA81000.10129025021317847No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGA13750.014.66545516
TACCGTC15600.014.5865397
AAGACGG20900.014.51674655
TATACCG3350.014.3582095
TAACGGC3500.014.27142836
CTTATAC39550.014.21997537
ACGGACC20200.014.1039618
CGTCGTA14700.014.09523810
GACGGAC20250.013.9777787
CGCAAGA22050.013.6757372
CAAGACG24150.013.6356114
ACCGTCG15900.013.6132078
CGAGCCG15650.013.47603915
TCTTATA63400.013.45189237
TCGTTTA13300.013.35338430
ATACCGT18600.013.3279576
CCGTCGT16250.013.329
GCGCAAG23150.013.0259181
TTAACGG3700.013.035
GTATTAG20950.012.98090651
Produced by FastQC (version 0.11.5)