FastQCFastQC Report
Thu 28 Apr 2016
ERR1042285.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042285.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8494424
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC473890.557883618712699No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT383710.4517198576383755No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG324010.38143845892317124No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA291530.3432016108449496No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT264580.3114749157800458No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT244720.2880948725893598No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT212820.2505408253696778No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG170880.20116725984010217No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC167970.1977414831188083No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG167570.19727058597498784No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT161690.190348397960827No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG149650.17617439393183104No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA123860.14581330058400663No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG95520.11245023794432676No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC92320.10868306079376305No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA91090.10723505207651514No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC89600.1054809602157839No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT85570.10073667149179273No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC85440.10058362992005108No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG5550.016.33333435
TATACTG10500.015.5047625
TAACGGC5850.015.49572736
TATAACG8700.015.0977022
AAGACGG24600.014.9654475
TCGTTTA16750.014.13731330
ATTAGAG16250.013.8892313
ATAACGC28750.013.7060873
CGAACGT7700.013.6948054
GTATTAG30000.013.6283331
TATCACG9350.013.4545452
TAATACT18700.013.3556154
TAGACTA6850.013.2335775
TCGAACG8000.013.1812513
CGTTATA2254.0745363E-1013.1555562
GACGGAC26300.013.1539937
ACGGACC26800.013.1156728
GTATAAC13400.013.1156721
TATCTAG21350.012.9976581
AAGTACT35300.012.9447594
Produced by FastQC (version 0.11.5)