FastQCFastQC Report
Thu 28 Apr 2016
ERR1042284.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042284.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2829864
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC564851.9960323181608726No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG367451.2984722940749096No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT360981.2756090045316664No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA347791.2289989907642205No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT238250.8419132509548162No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT219960.7772811696957875No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC214080.7565027859996099No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG180710.638581924785078No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT175640.6206658694552105No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG171380.6056121424916533No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT170870.6038099357424951No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG170130.6011949690868537No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA120110.4244373581203903No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC100990.35687227372057456No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA93790.3314293549089285No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT93490.3303692332917766No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC93420.3301218715811078No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC90880.32114617522255484No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG88390.31234716580019395No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT84340.298035523968643No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82260.29068534742305635No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA77140.2725926051569969No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG73840.26093126736832584No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68450.24188441564682966No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC67180.2373965674675532No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT67000.23676049449726208No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC66970.23665448233554687No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT63890.2257705670661205No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT62380.2204346215931225No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC61130.2160174481883228No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG56710.20039832302895122No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT55970.1977833563733098No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA55380.19569845052624435No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT48840.17258779927233253No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT48200.1703262064890751No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG48110.1700081700039295No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47680.16848866235267843No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC47640.16834731280372484No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47000.16608572002046743No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG45550.16096179887089979No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG45230.1598310024792711No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG44020.155555178623425No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT39740.14043077688539096No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA39180.1384518832000407No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT39110.13820452148937193No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC37910.13396403502076423No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT36750.12986489810111018No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCAT33810.11947570625302135No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA33690.11905165760616057No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA32600.11519988239717528No Hit
GAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA32530.11495252068650649No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT31410.11099473331580599No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT31140.11004062386036925No Hit
CGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30460.10763768152815825No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT30150.10654222252376794No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG29800.10530541397042402No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA29620.10466934100013287No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG28890.10208971173172987No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATA1300.025.6153852
CGAATTA450.00382717120.55555515
GATACAT4350.020.4137941
TACGTAG951.6782724E-719.47368214
AAAAGTA17450.016.9627512
GTACGTA1201.0427539E-716.95833213
AAAAAGT17400.016.9051721
CGTTAAT1005.8838996E-616.6500021
CGTAGCG904.449655E-516.44444515
GTTATAC2700.016.4444453
AAAGTAC18500.015.93
TGACCCG1751.3278623E-1015.85714313
GGACCGT700.002593749415.8571436
GTACTCC14100.015.7446836
GTATAAC6850.015.6642331
TACGGCT957.0655115E-515.5789464
AGTACTC20200.015.4777235
TAAGTTC2400.015.4166663
TGCGCGC1455.3541953E-815.31034519
TATAACG6050.015.2892562
Produced by FastQC (version 0.11.5)