FastQCFastQC Report
Thu 28 Apr 2016
ERR1042282.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042282.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4386947
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT445421.0153302513114475No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT281760.6422689856977984No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT258040.5881994927223876No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT169680.38678379292022447No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC154950.3532069113212446No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA118700.2705754138356356No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG118550.27023349039776406No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG76150.17358313195942415No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72890.16615199590968388No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG71560.1631202747605567No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT70160.15992898934042285No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA68750.1567149090244309No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66120.15071985141375083No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG62780.14310635619714576No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT59300.1351737324385273No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA43950.10018356729634527No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG12350.017.5263165
TTAACGG2100.016.73809435
GCGCAAG13850.016.1624551
CGCAAGA13550.015.8376392
ACGGACC13200.015.696978
GACGGAC13450.015.4052057
CGGACCA13600.015.3713249
CAAGACG15900.015.0094354
TAACGGC2350.014.95744736
CGTTATA1508.117604E-814.82
GGTTATC5200.014.5865382
AGACGGA14500.014.4172416
GCAAGAC18800.013.8753
CGAACGA6700.013.8059716
TATACTG5400.013.7037045
TATTAGA6750.013.7037032
TATCGGC950.001246052313.63157915
CGTCGTA9150.013.14207610
TTAGACT4100.013.0853654
TCGCGTG850.00941025213.05882412
Produced by FastQC (version 0.11.5)