FastQCFastQC Report
Thu 28 Apr 2016
ERR1042281.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042281.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6273774
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC1166361.8591042648332567No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG814211.297799378810904No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA732621.1677500655905042No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT634011.0105719460088936No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT403120.6425478507832766No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC395110.6297804160621661No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT393410.627070723299883No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG392480.6255883619652223No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG387990.6184315851989568No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG376030.5993680996478356No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT375480.598491434342391No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT321410.512307265132598No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA288980.46061589084975013No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC234350.3735391169653226No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC218590.3484186711220391No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG214250.34150098489362224No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT213470.34025771409681No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC203710.3247008897674669No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT194320.30973382209815015No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA173170.2760220562615102No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC171290.27302545485380886No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA157830.2515710639242026No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG156550.24953082466789528No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT155310.24755434288834757No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT149930.23897896226418103No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT145210.2314555800065479No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC144450.23024418794811544No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143310.22842709986046675No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC141020.2247769843159795No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG133980.21355566840628942No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127470.20317913906366408No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG120740.19245194359886092No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA118300.18856273751652514No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG118030.18813237454839782No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC117090.18663407384454717No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT113350.1806727497675243No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG105400.16800095126155326No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT104570.1666779836187915No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102640.16360168536514066No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT94990.15140806793486664No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG89170.14213135506634444No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT87920.14013893391760684No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC87110.1388478450132249No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT84270.13432106416329312No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT82940.13220112806103632No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG81160.129363920345234No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA77150.1229722333000838No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT76770.12236653727086759No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73310.11685151553116194No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT72880.11616612265599623No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT66490.10598086574364968No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT65290.10406814144086159No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA65100.10376529342625347No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG64070.1021235383996937No Hit
GAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA63550.10129469120181886No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA62930.10030645031204503No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGTA43000.018.2848852
AAAAAGT42600.018.1960091
CGTTATA2350.018.1063842
GTATAAC12950.017.7142851
CACGCAG9100.017.4835175
TATAACG12250.016.7632662
CGCAAGA10500.015.5047622
AAGACGG9400.015.3510655
AAAGTAC51400.015.3326853
TAACGGC2300.015.28260936
TTAACGG2300.015.28260935
GTACTCC30200.015.2533136
AGTACTC53200.015.1268795
GATACAT8300.014.9337351
AAGTACT56500.014.9309734
TATCACG10550.014.9052122
ACGGACC9850.014.6497458
GACGGAC9600.014.6458347
ATAACGC20950.014.48213
TACGACG7950.014.1949695
Produced by FastQC (version 0.11.5)