FastQCFastQC Report
Thu 28 Apr 2016
ERR1042280.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042280.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1815734
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT64850.35715583890591907No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43140.23758986723826286No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT41530.2287229296802285No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC37390.20592223310242577No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA30430.16759062726148213No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG24110.13278376678522294No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT22810.1256241277632076No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT21960.12094282532573604No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG20870.11493974337650781No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG19500.1073945853302301No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT19100.1051916194773023No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATCG500.00703641518.59
TGCGAAC500.00703641518.529
CGAACGT2450.016.6122444
ATGCGTG2600.016.3653857
GGTTATC3200.016.18752
AAGACGG6900.016.0869565
TACACCG700.002593269315.8571435
TCTATAC1659.749783E-1015.696973
GTCGGGA1557.215931E-915.5161292
GTATACT1353.9755287E-715.0740734
CAAGACG7400.015.04
TTATCTA4000.014.84
TTATCGA750.004105624714.810
GATGCGT3150.014.682546
TATAACG1405.999864E-714.5357152
CGTCGTA4350.014.4597710
TCGTTTA3850.014.41558430
AAATCGC3650.014.19178114
CAAATCG3650.014.19178113
CGCAAGA7300.014.1917812
Produced by FastQC (version 0.11.5)