FastQCFastQC Report
Thu 28 Apr 2016
ERR1042278.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042278.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10060968
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT139860.13901246878034001No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT118660.11794093769108499No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT102990.10236589560765923No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG32800.015.0594525
TATCTAG30500.014.5573771
GACGGAC32300.014.5479887
ACGGACC32900.014.5075988
TACCGTC20100.014.3582097
TCGTTTA19800.013.73484930
ACCGTCG20400.013.6936278
CAAGACG38900.013.64914
ATTAGAG21700.013.6405533
TTAGAGT19400.013.6365984
AGACGGA35300.013.6260626
CGTTATA2051.4279067E-913.5365862
CGGACCA35700.013.5252099
CGAACGT10400.013.5192314
GGTTATC18900.013.50793652
TATACTG15700.013.4331215
GTATTAG39950.013.3829791
CGCAAGA36800.013.3722822
TTAACGG5000.013.3235
CGAACGA14400.013.23263816
Produced by FastQC (version 0.11.5)