FastQCFastQC Report
Thu 28 Apr 2016
ERR1042277.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042277.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9801490
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC310760.3170538356923284No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT284060.2898130794399627No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT212720.2170282273409451No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG201110.20518308950986022No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194510.19844941942500577No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA182160.18584929434198272No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT179100.18272732002991382No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG113850.1161558089637392No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC113450.11574770774647528No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG108700.1109015057914664No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT101620.10367811424589526No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT500.007038570518.49999814
TATAACG6600.016.2575762
TTAACGG6450.015.2015535
TAGACCG1551.2130658E-714.3225815
AAGACGG26300.014.20912555
TATACTG11850.014.2067515
TAACGGC7300.013.68493236
GTATTAG37050.013.6315781
GACGGAC26200.013.4866427
ACGGACC27100.013.38007458
TAATACT26650.013.328334
TACCGTC20800.012.9855777
ATACCGT23450.012.8592746
CGAACTA7350.012.83673524
AGTACCG14000.012.6857145
ATAACGC27700.012.6227443
ACCGTCG20800.012.5408658
TATCTAG26350.012.3567371
GTATAAC11600.012.2801731
CACTCTA25700.012.2373539
Produced by FastQC (version 0.11.5)