FastQCFastQC Report
Thu 28 Apr 2016
ERR1042276.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042276.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5147811
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC274500.5332363600761566No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT227590.442110248414326No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA170110.330451137386357No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161540.31380328454172074No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG159960.3107340187897341No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT155420.30191473618592446No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT129080.25074735649774244No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC109320.21236210886530216No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG104430.20286292562022964No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG100930.19606391920760105No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT92400.1794937692933948No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA78110.15173439739726266No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG73550.14287626332823794No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG71350.13860260215458572No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA66040.12828753813999774No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56260.10928917164985273No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC54450.10577311404789337No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG801.6184522E-518.55
TATAACG5900.015.3644082
CGAACGA6050.014.67768716
GTATAAC7300.014.4452061
AGTACCG7350.014.3469385
ATACCGT10350.014.1207736
AAGACGG12650.013.8932815
TACCGTC9100.013.8241777
TAGTACC8150.013.6196324
TTAACGG4350.013.60919535
TAACGAA7100.013.54929613
ACGGACC12800.013.4414068
CGTCGTA9100.013.41758310
TATCTAG12300.013.3861791
ACCGTCG9150.013.3442618
GACGGAC13100.013.2748097
CGTATAC1409.573452E-613.2142853
GTATTAG18750.013.1226671
CCGTCGT9400.012.9893629
TAATACT13050.012.9003834
Produced by FastQC (version 0.11.5)