FastQCFastQC Report
Thu 28 Apr 2016
ERR1042275.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042275.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8353595
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC371890.44518557579102175No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT284400.3404522244614444No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG238120.2850509271756651No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA223540.2675973637697303No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT196650.23540762988868863No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188480.22562740951650156No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT166490.1993034136799785No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC136820.16378577127572022No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG124410.14892989186092934No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG122140.14621249893010135No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT116820.13984398333890977No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG107500.1286871101603561No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA93400.11180814966490475No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG88890.106409276485154No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG7550.017.397352
TAACGGC7750.015.27741836
TTAACGG7900.014.51898735
TATACTG11000.014.4636365
GTATTAG34650.014.41558551
AAGACGG22500.014.1422215
TAACGCA9700.014.1134024
ATTAGAG19100.014.0445023
ATAACGC24750.013.8282833
CGAACGA11500.013.67391316
ACGAACG11400.013.63157815
ACGGACC22950.013.6230948
GACGGAC21900.013.5159837
GTATAAC12000.013.4124991
CGAGCCG23350.013.38972115
TATTAGA16250.013.322
TCTATAC9300.013.1290323
TTAGAGT13700.013.098544
TACCGTC15050.012.9069767
TCGAACG9100.012.8076923
Produced by FastQC (version 0.11.5)