FastQCFastQC Report
Thu 28 Apr 2016
ERR1042274.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042274.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4967884
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC370700.7461929465341783No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT253990.5112639506075424No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA235250.47354165274390464No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG230310.4635977812686448No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173910.3500685603770136No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT163140.3283893102173884No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC141360.2845477068305137No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG135770.2732954312137723No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG134570.27087991587565247No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT129630.2609360444003926No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT124630.25087139715822676No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG104850.2110556526682185No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA95730.19269773609850793No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA75270.15151319958356516No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG70820.14255566353803753No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC69700.14030118255579238No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC68890.1386707097025615No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC64410.12965278577358086No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT63300.12741843408582004No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59720.12021214666042927No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT58880.11852128592374539No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53460.10761120831323759No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG50890.10243797963076433No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG50420.10149190279000074No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA50240.10112957548928277No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG502.7038544E-422.1999995
TTAACGG4250.017.41176635
TATAACG6250.016.282
AAGACGG12900.016.0620165
TAACGGC4500.016.03333336
CCTATCG957.067227E-515.5789488
GTATTAG20600.014.9975721
CTACGCT750.00410695814.84
GTATAAC8500.014.81
ACCGTCG9450.014.2910058
ATACCGT10700.014.1775696
TACCGTC10200.014.14705857
CGTCGTA9600.014.06770910
CGTTATA1854.9112714E-914.0000012
CGCAAGA14450.013.9550182
GACGGAC13800.013.9420297
TATTAGA10000.013.8752
CGCTAGG950.001246093113.63157934
AGTACCG6950.013.5755415
ACGGACC14200.013.5492958
Produced by FastQC (version 0.11.5)