FastQCFastQC Report
Thu 28 Apr 2016
ERR1042273.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042273.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7816452
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT808971.0349580602554713No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT510860.6535701875991818No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT456430.583935012970079No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC452350.5787152534167677No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT416280.5325689967775661No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG315760.40396845013568816No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA280830.35928065572461776No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA219440.28074118538692494No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG184870.23651395799526437No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG178290.22809581636271803No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT155990.1995662482159425No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151070.19327183228400815No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG146270.18713093869187708No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC134710.17234161995749478No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA132830.16993643663391012No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127390.16297675722949492No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT107940.13809334465304718No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT104230.13334694564746255No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101070.1293041906993096No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC89960.1150905807391896No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC89210.11413106611541912No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT86420.11056167171499293No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83770.10717138671100392No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC83290.10655729735179081No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA81670.10448474576444658No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT78910.10095373194897123No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG26700.017.183525
CAAGACG28000.017.0464294
TATAACG12200.016.6803282
GACGGAC27500.016.4818177
ACGGACC27400.016.4744538
CGGACCA28750.016.0226089
AGACGGA28300.015.88515956
CGAGCCG25300.015.57509815
GCGCAAG29400.015.3537411
CGCAAGA30650.015.2707992
CGAACGA14600.014.95205516
GTAAACG20000.014.70750127
AGTACTC32350.014.6970635
GTATAAC15850.014.5899051
GCAAGAC35150.014.3684213
GGTTATC15750.014.3301582
TATCTAG27400.014.3138691
ACGAACG15300.014.26797415
TACCGCA26650.014.23076928
TAAACGC20500.014.0780528
Produced by FastQC (version 0.11.5)