FastQCFastQC Report
Thu 28 Apr 2016
ERR1042272.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042272.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1896764
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT155160.8180248043509893No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC127270.6709848984902708No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106500.5614826093283086No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA91560.48271687990704165No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT91240.4810297960104684No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG83160.438430927621992No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT68680.3620903813020492No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG55350.2918127927354167No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC54410.2868569837892326No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG51440.2711987363741615No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT48510.25575137444616197No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA40260.21225624273763102No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG38590.203451773652389No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35060.18484112941831457No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA34210.18035981281804167No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27850.1468290203736469No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC25560.1347558262387941No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC24990.13175070804802286No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT23940.12621496401239163No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC23770.12531870069233705No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT23470.12373705953929956No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT22350.11783226590129293No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA21760.11472170496698587No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21270.11213835775035798No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC19270.10159408339677473No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC19060.10048693458964847No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG4050.020.5555555
TAATCGG555.144789E-420.18181816
TACCGTC3000.019.7333327
TTAGACT1552.1827873E-1117.9032254
CGTCGTA3150.017.6190510
ACCGTCG3150.017.619058
CGCAAGA4750.017.5263162
ATTAGAG3650.017.2328763
ATACCGT3650.017.2328766
GGATCGT650.001580446417.0769236
CCGTCGT3250.017.0769239
TTAACGG1201.04222636E-716.95833235
GACGGAC4700.016.9255337
ACGGACC5050.016.8514868
ATTAGAC1655.4569682E-1116.8181823
CAAGACG5300.016.7547174
CGGACCA5300.016.405669
AGACGGA5300.016.0566046
TCTACAC2550.015.9607843
TATTAGA3300.015.6969712
Produced by FastQC (version 0.11.5)