FastQCFastQC Report
Thu 28 Apr 2016
ERR1042271.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042271.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3194779
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT246080.7702567219829602No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT192030.6010744405168558No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164470.5148086925574508No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT81490.2550724165896921No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA63470.19866788907777344No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC62450.1954751799733252No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48560.15199799422745672No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG46870.14670811345636114No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT46390.14520566211309138No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45040.1409800177101452No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG38690.12110383848147244No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG36330.11371678604372946No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG36160.11318466785965475No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT35170.1100858619641609No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT35000.10955374378008619No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGTC3100.017.90322710
TAATCCG852.7255732E-517.4117645
AAGACGG10850.017.3917055
CGAACGT3850.017.2987024
CGCAAGA11300.017.1902662
GCGCAAG10900.016.9724771
TACCGTC7250.016.3310367
ACGGACC11250.015.9511128
CAAGACG12750.015.67058854
TCGAACG4250.015.67058853
CGTCGTA7100.015.63380310
GACGGAC12100.015.5950427
ACCGTCG7600.015.0921048
AACCGCG4350.014.8850587
AACGTCT5100.014.8725496
TGTCGTA1152.2121689E-514.47826137
CCGTCGT7950.014.4276729
ATACCGT9000.014.3888896
ACGTCTG4900.014.3469387
TATCTAG7100.014.3309861
Produced by FastQC (version 0.11.5)