FastQCFastQC Report
Thu 28 Apr 2016
ERR1042270.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042270.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2378114
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT88830.3735312941263539No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT83820.35246417959778215No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73580.3094048477070485No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT56420.23724682668703015No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC43780.18409546388440587No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA43720.1838431631116086No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT29900.1257298851106381No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG25380.10672322689324397No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACTA900.030.83333224
GCGAACT1603.45608E-1117.3437523
AAGCGCG752.0684382E-417.2666665
TACCGTC5900.016.6186457
AAGACGG15050.016.2259145
GCGTCGT700.002593586515.85714225
GACGGAC14400.015.6736117
CGCAAGA15500.015.5161292
ACGGACC14700.015.4795928
AGACGGA15500.015.2774186
CCGTCGT5600.015.1964289
CGAACGT2450.015.1020414
CGATAGA2700.015.07407537
ACCGTCG5650.015.0619488
GCGCAAG16000.015.0312511
CGGACCA15550.014.9903559
ATACCGT6800.014.9632366
CGTCGTA5600.014.86607110
ACGTCTG2750.014.87
TAGTACC5500.014.84
Produced by FastQC (version 0.11.5)