FastQCFastQC Report
Thu 28 Apr 2016
ERR1042269.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042269.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7325697
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT349000.47640518028523426No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC301610.4117150900453568No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT241640.3298525723900402No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT235920.32204444164152574No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG210710.28763133391948914No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA200960.2743220201436123No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT172370.23529501697927171No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG124690.17020905997067584No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG123880.16910336313391067No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC116360.15883812830369587No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT114830.15674958983425058No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG107390.14659355962988913No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA83810.11440549615961457No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82130.11211219901669425No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA81050.11063793656767404No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCG13450.014.7174728
TACCGTC14200.014.591557
ATACCGT16500.014.3515166
CGTCGTA13550.014.335793510
TATAACG6850.014.31386852
TATACTG11050.014.2307695
CCGTCGT13950.014.0573479
CGAACGA8900.013.719116
ACGAACG9100.013.41758315
TTAACGG4850.013.35051535
AAGACGG19750.013.2075955
TAATACT16550.013.1903324
CTAGTAC3150.012.9206353
CGAACGT6500.012.8076934
ATTAGAG15700.012.608283
AGTACCG9250.012.65
TCGAACG6650.012.5187973
TAACGGC5250.012.33333336
TAACGCA9000.012.3333324
TAGTACC10700.012.2757014
Produced by FastQC (version 0.11.5)