FastQCFastQC Report
Thu 28 Apr 2016
ERR1042268.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042268.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences678181
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT40880.6027889309786031No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35780.5275877678672802No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT34630.510630642851982No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA29990.4422123297467785No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG21500.31702451115557645No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT20510.30242663831631966No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC19400.2860593263450318No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG18890.27853921003389953No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG13460.19847208930949112No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC12470.18387421647023436No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT10620.1565953631847545No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG9900.14597872839256776No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9860.14538891534855738No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC9830.1449465555655496No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT9510.14022805121346663No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT9460.13949078490845362No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8890.1310859490313058No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC7940.1170778892360594No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG7530.11103230553495307No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG7180.10587144139986228No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6900.10174275009178965No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGTA250.00549477429.630
GTACGTA502.7005194E-422.213
CTACGCA450.003824214520.5555579
CGTGTCG450.003824214520.55555727
TACCGTC1001.2874807E-820.357
CTCGCCG555.140106E-420.18181813
CCGTCGT1052.255365E-819.380959
CGTCGTA1052.255365E-819.3809510
ACCGTCG1052.255365E-819.380958
TTAATCG500.007031980418.537
ATACTCT701.21826226E-418.56
ACCTAGC500.007031980418.57
TGCCTCG801.6150778E-518.510
ACATCGT609.2314935E-418.58
TGTACGT609.2314935E-418.512
TATAACT801.6150778E-518.54
ATACCGT1501.2732926E-1118.56
TAGTACA500.007031980418.54
TCACGTA609.2314935E-418.525
AAGACGG2450.018.1224485
Produced by FastQC (version 0.11.5)