FastQCFastQC Report
Thu 28 Apr 2016
ERR1042267.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042267.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9027339
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC191870.2125432533330143No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT190710.21125826780184062No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT145130.16076719839589496No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132490.14676528709069195No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT128540.142389689807816No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG121530.13462438931339568No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA115070.12746834920013528No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG7250.014.835
TAACGGC7400.014.7536
TATAACG4700.014.5638282
GTATTAG31150.014.3130011
TATACTG17250.014.0492765
AGTACCG12650.013.7470365
ATTAGAG22550.013.6186263
TACCGTC16750.013.5850747
TAACGCA6000.013.5666674
TATACCG3150.013.5079375
ACCGTCG16400.013.42377958
TAATACT25850.013.3829784
GGGGTTA14550.013.3505146
AAGACGG24700.013.33198455
CGTCGTA15800.013.23101210
TAGACTA9050.013.0828725
ATACCGT19600.013.025516
CCGTCGT16650.012.8888899
TAGTACC14400.012.8472224
TACGGGT2451.1459633E-1012.8367344
Produced by FastQC (version 0.11.5)