FastQCFastQC Report
Thu 28 Apr 2016
ERR1042266.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042266.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences993484
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT37800.38047920248338174No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35890.3612539306118669No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT35060.3528994930970202No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA22080.2222481690696579No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT20650.20785437913444002No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT15180.1527956162353898No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG14830.14927266065683997No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC14040.14132084663668465No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG13040.1312552592693994No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG10810.10880899944035334No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10530.10599063497751349No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT10010.10075652954652517No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACTA555.142418E-420.1818185
TATACTG1202.382876E-1020.0416685
ATAGTGT1551.8189894E-1219.0967756
TCGCGTA1950.018.974369
ACGGACC6200.018.58
TACTCCG1002.8739487E-718.4999985
CGCAAGA6500.018.2153842
GCGCAAG6550.018.0763361
CGGACCA6300.017.9126999
GACGGAC6500.017.6461547
CGCGTAA2100.017.61904710
TTATCTA2350.017.3191494
GTCGCGT2150.017.2093038
TAATATC650.001579730517.0769214
CGTGTTA650.001579730517.07692134
TAGGCCG650.001579730517.0769215
CTTATGC1201.040753E-716.9583343
GGTTATC2300.016.8913042
GCGTAAC2200.016.81818211
AGACGGA7400.016.56
Produced by FastQC (version 0.11.5)