FastQCFastQC Report
Thu 28 Apr 2016
ERR1042265.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042265.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6892601
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT146230.21215503407204334No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC123840.17967092538796312No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT111040.16110028710496951No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA97320.14119488419538576No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95780.13896060427696308No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG91290.13244637256675673No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT83340.12091226519567867No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTTA14000.015.32857130
TAGAGTG15600.015.1794875
TACCGTC16900.014.9970427
GGTTATC14550.014.8762882
TATACCG2400.014.6458335
TTAGAGT16000.014.5687494
ACCGTCG16650.014.4444458
TATACTG11900.014.14705855
ATTAGAG19100.014.0445023
CGTCGTA16600.014.04216910
CGAACTA3450.013.94202924
AAGACGG24850.013.9215295
CCGTCGT16900.013.9023689
ATACCGT19250.013.8389616
AGGTTAT16350.013.6911311
TTAGCGA2304.0017767E-1113.67391327
TATCTAG22900.013.4912671
GACGGAC24600.013.3861797
TATCCCG4300.013.3372095
ATAACGC20850.013.3093533
Produced by FastQC (version 0.11.5)