FastQCFastQC Report
Thu 28 Apr 2016
ERR1042264.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042264.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1456582
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG101600.6975233800774691No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG101540.6971114568215178No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG89310.6131477664834523No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT81010.5561650494101945No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTG58970.4048519067240979No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAG55760.38281401253070546No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTT55370.38013651136702226No Hit
ACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGA51680.3548032311260197No Hit
AAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAA50090.3438872648433113No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTA48880.33558014584829415No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG39730.27276184931572683No Hit
GTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAG38900.267063577608401No Hit
GGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGT37370.2565595345816439No Hit
CAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGC36680.2518224171382044No Hit
TATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTAC30350.2083645136353463No Hit
ACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAG27630.1896906593655558No Hit
GTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGA27370.18790565858976702No Hit
CAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCA23830.16360218648864258No Hit
ACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGT23370.16044410819301627No Hit
GCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACG22960.15762929927734928No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGT20230.13888679113156693No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTAT20120.13813159849565626No Hit
ACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATAC19250.132158711284363No Hit
CCTATACCCAGGTCGGACGACCGATTTGCACGTCAGGACCGCT19240.1320900574083711No Hit
AAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAAC19140.13140351864845234No Hit
GAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAA18840.12934390236869603No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC18310.12570524694112656No Hit
CGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATC17540.12041889848975204No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG15970.10964023995902736No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTG15260.10476581476360411No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT14960.10270619848384781No Hit
GGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGT14810.10167639034396965No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGC450.003826291320.55555735
GCTCCGA4900.020.01020429
TACATTA500.007035762518.55
CGCTATC609.238432E-418.512
CATACGG701.2194814E-418.534
ATCATCG701.2194814E-418.57
TATCCTA500.007035762518.532
TTAGGTT1351.1532393E-917.8148164
GTCGCCC5250.017.26666837
GTAAGAT1851.8189894E-1217.03
AGGTCGC5450.016.97247735
TCGGACG4950.016.44444513
CCGAGGT5650.016.37168132
CCATACG803.3833424E-416.187533
ACTGCGT3450.016.0869568
TCCGAGG6100.016.0737731
TAGTACT1059.346448E-615.8571424
GTCGGAC5150.015.80582612
GGGTAAG3050.015.7704911
TTTACTT2003.6379788E-1215.72523
Produced by FastQC (version 0.11.5)