FastQCFastQC Report
Thu 28 Apr 2016
ERR1042263.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042263.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4942669
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG206190.4171632775733111No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT198180.40095745840961633No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG139800.28284313596560884No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG132370.26781077187244384No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGT130790.2646141184044491No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT122050.24693136441060487No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTG119020.24080107326628586No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT116690.2360870210001924No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG115300.23327477522771603No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTT108990.22050839333971178No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC95550.19331660687778202No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG93120.18840023477194204No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG83270.16847173055691164No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT82990.16790523500562146No Hit
CCTATACCCAGGTCGGACGACCGATTTGCACGTCAGGACCGCT80030.16191656774912502No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG72720.14712698746365577No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC68230.13804282665903786No Hit
GGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGT63180.12782567475183954No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT62520.12649036380951265No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC60780.1229699985979235No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG57940.11722411514912287No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCA56500.11431070945677325No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC56290.11388583779330559No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAG55710.1127123827227759No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGT55060.11139730376442364No Hit
CGTCAGGACCGCTACGGACCTCCACCAGAGTTTCCTCTGGCTT54920.11111405598877852No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGAC53200.10763415474513871No Hit
GTCCTGACGTGCAAATCGGTCGTCCGACCTGGGTATAGGGGCG53150.10753299482526546No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT53000.10722951506564572No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTA52600.1064202357066597No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG52400.1060155960271667No Hit
GCTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCG51980.1051658527002314No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTG51000.10318311827071569No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA50660.10249523081557758No Hit
GATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTT49650.1004518004341379No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC9300.023.2741933
TTATACA10700.020.2289712
TACACAT13750.017.3563635
ATCTCCG14100.016.6631210
ATCTCGT12900.016.63565837
AATACTG23050.016.3731025
TATTAGA27950.016.2826482
ATACTGG22400.016.18756
TACTGGT22000.016.0613637
GTAAACA20300.016.0394136
ATACACA16150.015.9226014
GGTAAAC20650.015.85714235
TTGGTAA20850.015.79376533
GTGACAC26650.015.68855524
GATGCTA21850.015.57894714
GTATTAG34750.015.4388491
AATCGTC1802.0190782E-1015.41666728
ACCGCCT27050.015.38816912
TAAACAG21550.015.36658937
TCTCCGA14350.015.34146211
Produced by FastQC (version 0.11.5)