FastQCFastQC Report
Thu 28 Apr 2016
ERR1042262.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042262.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3370781
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT163880.4861781290448712No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT160720.47680344703497496No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT111730.3314662091663623No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG110070.3265415344396447No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC89570.26572476823620406No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG79770.23665138731943725No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC77520.22997637639466934No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT75880.22511103509839414No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG73360.2176350228626541No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT72700.2156770196580555No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG67360.19983499372993974No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61920.18369630064961207No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA58440.17337228375263775No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC57290.16996061150220082No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG54490.16165393124026747No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG51810.15370325156098838No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG51650.15322858411744933No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC50130.14871924340382836No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCA48580.14412090254454382No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT48070.1426079000682631No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTT47920.14216289933994528No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA46130.13685255731535215No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC46130.13685255731535215No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGT45950.1363185564413707No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG45780.13581422228261048No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGAC45510.13501322097163831No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC44160.1310082144167776No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG44050.1306818805493445No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG42560.1262615399813871No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG42370.12569787239218447No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTG41750.12385853604847066No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC40930.12142586540033304No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAAC40400.11985352949360993No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTG39910.11839986044777161No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT39890.11834052701732922No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT39770.11798452643467493No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGA39450.11703519154759683No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG39270.11650119067361542No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACA39030.11578918950830683No Hit
GTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGA38440.11403885331025658No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTT38330.11371251944282348No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGG37770.1120511833904368No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT36810.10920317872920252No Hit
GTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTT36460.10816484369646086No Hit
CCTAAGAACAGCTAAAAGAGCACACCCGTCTATGTAGCAAAAT35100.10413017042637893No Hit
ATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCA35080.10407083699593654No Hit
GGGTAAGATTTGCCGAGTTCCTTTTACTTTTTTTAACCTTTCC35050.10398183685027297No Hit
GGCTTATGCGGAGGAGAATGTTTTCATGTTACTTATACTAACA34520.10240950094354989No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT34410.10208316707611678No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCAT34200.10146016605647176No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGT5650.024.88495637
CATCTCG6250.022.79236
TATACAC6900.021.7173923
CCCACGA6400.021.39062719
CGAGCCC6650.021.14285915
ATCTCCG7500.020.22666710
GCCCACG7050.020.20567318
ACGAGAC6850.019.98540122
TCTCCGA7300.019.51369911
TACACAT10100.018.6831685
CTACATC7850.018.38216433
GTGACAC21800.017.65137724
TTAACGG21400.017.3761735
TAACGGC21200.017.36556636
GACCTCT8300.017.1626526
ATTAGAG23550.017.1252653
AGTGACA22650.017.0706423
CACATGT22750.016.99560528
TATTAGA25050.016.9860292
GGTAAAC26050.016.9731335
Produced by FastQC (version 0.11.5)