FastQCFastQC Report
Thu 28 Apr 2016
ERR1042260.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042260.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1591485
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG166711.0475122291444783No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG163331.0262742030242196No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG154000.9676497108046886No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT153230.9628114622506653No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAG83120.5222795062473099No Hit
AAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAA75080.47176065121568844No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTA69480.4365733890046089No Hit
GGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGT67080.42149313377128905No Hit
ACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGA65750.4131361589961577No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTG62350.39177246408228794No Hit
GTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAG56660.35601969229995883No Hit
TATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTAC53460.3359126853221991No Hit
GTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGA49620.3117842769488874No Hit
ACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAG47720.2998457415558425No Hit
CAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGC44720.28099542251419274No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTT42610.26773736478823235No Hit
CAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCA35950.22588965651576987No Hit
AAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAAC35270.2216169175329959No Hit
GAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAA31640.19880803149259968No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGT30660.19265026060566076No Hit
ACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGT29770.18705799928997133No Hit
GCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACG27730.17423978234164947No Hit
CGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATC27010.1697157057716535No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG24620.15469828493513918No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTAT24500.1539442721734732No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT23900.15017420836514325No Hit
GTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTA23100.1451474566207033No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT18700.11750032202628363No Hit
GGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATC17820.1119708951073997No Hit
AACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGG17180.10794949371184773No Hit
AACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAA17070.10725831534698724No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAA609.238944E-418.510
GGGTATA650.001580295417.0769231
ACAGCGT1757.2759576E-1216.9142868
CGTGCGA957.062401E-515.57894810
TATACTC855.3657603E-415.2352945
TATACGG855.3657603E-415.2352945
ACCGTCG1101.4524867E-515.1363648
GGGTTAG1405.998518E-714.5357151
TACACTT1152.2107903E-514.478265
AGACCGT1304.4462613E-614.2307696
GGACCGT1051.6562625E-414.0952386
TATACAC2500.014.0600013
TTATACT1203.301927E-513.8750014
TATACTT1203.301927E-513.8750015
TGTATAC800.00630002613.8753
ACTCCGT1601.7844468E-713.8756
AGTACTA800.00630002613.8755
GTGTTAG1356.570295E-613.7037041
TACCGTC1102.45729E-413.4545457
AGTACTC4150.013.3734943
Produced by FastQC (version 0.11.5)