FastQCFastQC Report
Thu 28 Apr 2016
ERR1042259.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042259.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4106050
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT368900.8984303649492822No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT347840.8471401955650808No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT245380.5976059716759416No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG164420.40043350665481425No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145050.35325921506070307No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT138560.3374532701744986No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG121390.29563692599943986No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG95930.23363086177713377No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG95490.2325592722933233No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG95010.23139026558371187No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC93610.2279806626806785No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGT84770.20645145577866805No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG78200.19045067644086167No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTT76490.18628609003787094No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT75170.18307132158643952No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTG74160.18061153663496546No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG73630.17932075839310285No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC72080.17554584089331596No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGT65810.16027569074901668No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG63360.15430888566870837No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG61590.14999817342701624No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC61240.1491457727012579No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA57250.13942840442761292No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTT57170.139233569976011No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC52100.12688593660574032No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG51590.12564386697677818No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAG51400.12518113515422366No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC51130.12452356888006723No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA49840.12138186334798651No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT49810.12130880042863579No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG49730.12111396597703387No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTA49150.11970141620292007No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCA47950.11677889942889152No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAAC47920.1167058365095408No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGAC47570.11585343578378246No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC47420.1154881211870289No Hit
GGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGT45900.11178626660659272No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTG44970.10952131610672056No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGA44810.10913164720351676No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC44380.10808441202615653No Hit
CCTATACCCAGGTCGGACGACCGATTTGCACGTCAGGACCGCT43760.10657444502624176No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43280.10540543831663034No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT42540.10360321963931271No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT42340.10311613351030796No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACA42110.10255598446195248No Hit
GTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGA41980.10223937847809939No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT41790.10177664665554487No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGT10700.026.97196437
CATCTCG10900.026.13761536
CCCACGA11550.025.14718619
TATACAC11750.025.0340423
CGAGCCC11750.024.71914915
TCTCCGA11800.024.61440711
ATCTCCG12100.024.15702410
GCCCACG12200.023.95901518
ACGAGAC12150.023.60082222
GCATCTC12400.022.97580735
TACACAT14000.022.8607165
CGAGACT12600.022.75793623
ACACATC14750.022.1999996
CACGAGA13400.021.53731321
AGCATCT13650.021.00732634
ACATCTC14650.020.7098988
TTATACA14400.020.298612
ATACACA16100.019.7639754
CTGAGCA14650.019.69965731
AGCCCAC15500.018.85806517
Produced by FastQC (version 0.11.5)