FastQCFastQC Report
Thu 28 Apr 2016
ERR1042258.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042258.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1310675
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG28040.21393556755107102No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT25750.19646365422396855No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTG19010.14503976958437445No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG18950.14458199019589144No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG18330.13985160318156675No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGT17910.13664714746218554No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTT16140.12314265550193602No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC15240.11627596467469051No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG14980.11429225399126404No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCCCG555.1436224E-420.1818185
TTATAGA500.007035395618.54
TAACCCG701.2193629E-418.528
GTATACT701.2193629E-418.54
CGTTATT650.00158009417.0769232
CTATACC2150.016.3488374
CCCCTAT1708.54925E-1116.323531
GCCTTAA700.002592753915.8571421
ATTAGAG4600.015.6847813
TAGTACG1302.589568E-715.6538464
AACCCGT957.060883E-515.57894829
CTAGCAT3350.015.4626874
CCTCTAG4550.015.450551
TTTAAAC1201.9359068E-615.4166663
TAGGATA855.3648645E-415.2352944
TATACTG855.3648645E-415.2352945
ACTGCGT1101.4520978E-515.1363648
ACCAGTA3300.015.13636412
TACACTC1601.0966687E-815.031255
GTCTAAA1001.0935495E-414.81
Produced by FastQC (version 0.11.5)