FastQCFastQC Report
Thu 28 Apr 2016
ERR1042257.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042257.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3340671
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG47090.14095970540050187No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC42700.12781863284352157No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT38480.11518644008943113No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG38480.11518644008943113No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG33410.10000984832089121No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTAGA13950.016.577062
TATTCCG904.450042E-516.4444455
GTATTAG16000.015.8406241
ATTAGAG13100.015.3931293
TTAACGG12100.014.98347235
TTACGCA750.004106585414.79999927
GAACCGA1152.2122289E-514.478266
TAACGGC12550.014.29880436
TATACAC4250.013.9294123
ATCTCGT4150.013.81927737
TTAGACT2551.8189894E-1213.7843134
GTGTAAG5400.013.7037041
CACATGT14350.013.40766528
TTTAACG13800.013.40579734
TAATACT11800.013.3262714
GACACAT14500.013.01379326
GTAAGAT6150.012.9349593
TATACCG1307.005598E-512.8076935
TAGAACA4050.012.7901234
ACCGCCT15150.012.69967112
Produced by FastQC (version 0.11.5)