FastQCFastQC Report
Thu 28 Apr 2016
ERR1042255.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042255.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3705487
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT164710.44450297626195967No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT156760.4230483064709173No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT106450.2872766791517552No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG96560.26058653019157807No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT87300.2355965626110684No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66240.17876192791932613No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG61740.1666177752074154No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG58670.15833276435728963No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGT56410.15223370099530778No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG51970.14025147031955584No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTG49390.133288822764727No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTT47360.12781046054135395No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGT46630.12584040910142175No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGACG8550.021.20467823
GACGGAC8700.021.05172526
ACGGACT8950.020.87709627
TATCTCG9050.020.44198836
TATACAC10300.019.757283
CGGACTC9550.019.37172928
CCTATCT9550.018.98429534
TCCTATC9750.018.59487333
CTCCTAT10150.018.40886732
ATCTCGT9850.018.40609237
TACACAT11400.018.3377195
TCTCCGA10700.018.32710311
ACGAGAC10000.018.31522
TATACCG1451.546141E-1017.8620685
CACGAGA10550.017.710921
CCCACGA10450.017.70334819
ATCTCCG11100.017.510
CTATCTC10600.017.4528335
ACTCCTA10950.016.89497831
GCCCACG11800.016.30508418
Produced by FastQC (version 0.11.5)