FastQCFastQC Report
Thu 28 Apr 2016
ERR1042254.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042254.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences283694
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG18100.63801137845707No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG16980.5985322213370744No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG16480.5809075976227908No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT12790.45083787461137703No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAG10520.3708220829485291No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTA8640.3045534977828223No Hit
AAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAA8450.29785614077139455No Hit
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT8310.2929212461313951RNA PCR Primer, Index 26 (100% over 22bp)
GTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAG7830.27600160736568274No Hit
GGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGT7820.2756491148913971No Hit
ACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGA7750.2731816675713974No Hit
GTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGA6890.2428673147828294No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATG6440.22700515343997404No Hit
TATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTAC6110.21537290178854684No Hit
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCC5570.19633830817712045No Hit
ACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAG5020.17695122209140834No Hit
CAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGC4810.1695488801314092No Hit
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC4140.14593188435426904No Hit
GAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAA4040.14240695961141228No Hit
CAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCA3990.14064449723998393No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGT3940.13888203486855555No Hit
ACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGT3890.13711957249712717No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTG3820.13465212517712746No Hit
GCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACG3740.13183218538284208No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGT3550.12513482837141426No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3240.11420756166855836No Hit
CGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATC3160.11138762187427298No Hit
CTTAAACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGT3100.10927266702855894No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTT3090.10892017455427327No Hit
AAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAAC3080.1085676820799876No Hit
GCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATA3060.10786269713141625No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTAT2940.10363278743998816No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAAAC509.059606E-625.8999981
CCGTCTT1250.023.6837
TAAACAC551.8961167E-523.5454543
GCCGTCT1400.021.14285936
TTCCGTA1500.020.96666727
GACAGTA450.003818813520.5555557
CAGTTAC450.003818813520.5555551
CTTCCGT1550.020.29032326
TTATACA759.225483E-619.7333342
TATACAC759.225483E-619.7333343
AAGTACA609.2134526E-418.54
CAATATG609.2134526E-418.52
CTTGGAC500.007022144718.4999983
TGGATAA500.007022144718.49999833
TGTAATC953.5870507E-617.5263163
TCCTATT852.7114253E-517.41176417
CGCAATC852.7114253E-517.41176412
ACCCTCG752.0603577E-417.26666624
TGTAGAG650.001575971517.0769212
TACACAG650.001575971517.0769215
Produced by FastQC (version 0.11.5)