FastQCFastQC Report
Thu 28 Apr 2016
ERR1042253.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042253.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17943107
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT327300.18240988029553634No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT287650.16031225807213878No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT237010.13208972113915388No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG10550.015.60663535
ACGGACC29450.015.2020388
GACGGAC29550.015.1505927
TATACTG32200.014.7080755
TAACGGC11200.014.70089336
AAGACGG34300.014.3469395
TCTATAC30100.014.1362133
CTAGCGG18350.014.01362329
GTATTAG56350.013.9201421
TCTAGCG18850.013.44562328
CGGACCA33250.013.3533839
TTAGACT20000.013.3200014
CGAACGA14600.013.0513716
TAGACTA22150.013.02934555
TAACGCC19500.012.9974364
CGCAAGA37100.012.8153642
GGTATCA245350.012.7203991
TAATACT50350.012.6395234
ATAACGC24950.012.3827663
CTAATAC53100.012.3333333
Produced by FastQC (version 0.11.5)