FastQCFastQC Report
Thu 28 Apr 2016
ERR1042252.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042252.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21566600
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT560140.2597256869418453No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT449580.20846123171941797No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT381210.17675943356857363No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218570.10134652657349791No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA252000.019.2047621
ACGGACC35550.017.0689188
TAACGGC17350.017.0605236
TTAACGG17350.016.84726335
AAGACGG39900.016.5989975
GACGGAC37000.016.07
CGGACCA41000.014.9804899
TCTAGCG18900.014.97619128
CGCAAGA42250.014.9313612
TATACTG45600.014.9298245
CTAGCGG19100.014.81937129
TCTATAC35650.014.0112193
AGACGGA45850.013.9607426
TAACGCC23800.013.8361344
TACCGTC27050.013.541597
GTATAGG48200.013.4719921
GTATCAA359050.013.4685972
TCGTTTA23300.013.41845430
GTATTAG73100.013.3625171
ATACCGT31650.013.0347556
Produced by FastQC (version 0.11.5)