FastQCFastQC Report
Thu 28 Apr 2016
ERR1042251.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042251.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12719427
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT379330.29822884317037235No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT366750.2883384605297078No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT259790.20424662211591765No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185860.14612293462590728No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGGAC22750.016.589017
ACGGACC23100.016.5779238
TTAACGG11400.016.2280735
AAGACGG24300.016.1399175
TAACGGC11600.015.62931236
GTATTAG40800.015.4166671
CGCAAGA27200.014.6911762
CGGACCA26050.014.5585429
TCTAGCG13000.014.51538628
AGACGGA27550.014.5045376
CGAACGA12650.014.33201616
CTAGCGG13300.014.32706729
TATACTG23350.014.2612435
TTTAGCG8250.014.12727426
CGTCGTA15650.013.94888210
TAACGCC17000.013.9294114
CCGTCGT16500.013.5666689
TACCGTC17750.013.5492957
ACCGTCG17100.013.5233928
ATACCGT19900.013.4798996
Produced by FastQC (version 0.11.5)